GGEbiplot Pattern Explorer

Complete solution to biplot analysis, video game for scientists


Functionality

GGEbiplot functions

Functions for a 4-Way Dataset

Variable-by-Variable Biplot

Association Biplot

Genotype by env. biplot

Genotype by trait biplot

QTL mapping biplot

QTL by environment biplot

Gene expression biplot

Diallel cross data biplot

Host by pathogen biplot

3D-Biplot

Free User-friendly ANOVA

Breeder's Kit

 

 

Information 

Biplot Analysis Instructions

GGEbiplot Beta download

GGEbiplot License

Biplot Analysis Presentations

User's feedback

GGE biplot publications by users

GGE biplot publications

References on biplot analysis

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Some concepts


What is a biplot

The concept of biplot was first developed by Gabriel (1971). It  is a scatter plot that graphically displays both the entries (e.g., cultivars) and the testers (e.g., environments) of a two-way data. In breeding and genetics data, testers can also be traits, genetic markers, etc. When the two-way data is subjected to singular value decomposition, it is decomposed into three matrices: the singular value matrix, the entry eigenvector matrix, and the tester eigenvector matrix. The singular value matrix is a diagonal matrix, and can be somehow partitioned into the entry and tester eigenvector matrices. After singular values are partitioned, the positions of the entries in the biplot is defined by the entry eigenvector matrix and those of the testers by the tester eigenvector matrix.  

Properties of a biplot

A biplot graphically displays the two-way data and allows visualization of:

  • the interrelationship among the entries (e.g., genotypes), 

  • the interrelationship among testers (e.g., environments), and

  • the interaction between entries and testers.

See References for more in-depth information.

What is GGE

GGE stands for genotype main effect (G) plus genotype by environment interaction (GE), which is the only source of variation that is relevant to cultivar evaluation. Mathematically, GGE is the genotype by environment data matrix after the environment means are subtracted.

What is GGE biplot

A GGE biplot is a biplot that displays the GGE of a genotype by environment two-way data. The GGE biplot methodology originates from graphical analysis of multi-environment variety trials (MET) data, but is equally applicable to all other types of two-way data.

What are entries and testers

Entry and tester are the two factors in a two-way data. Entry is the factor to be tested, and tester is the factor used to test. An entry is a level of the entry factor, and a tester is a level of the tester factor. In genotype by environment data, genotypes are entries and environments are testers. In genotype by trait data, genotypes are entries and traits are testers. In genotype by genetic marker data, genotypes are entries and markers are testers. In convention, entries are presented as rows, and testers as columns in a data matrix.

Here is a biplot generated by GGEbiplot. It has 18 cultivars (in blue) and 9 environments (in red).
 

 


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